Cell adhesion with extracellular matrix is an important process for cellular mechanics. In integrin-mediated and actin-dependent adhesions, tractions are generated over time. The dynamics of tractions applied during cell adhesion may contain important information about cell status. In certain types of isolated cardiac cells, the dynamics of the tractions applied during cell adhesion is a cyclic and spontaneous contraction. This research is limited to studying the spontaneous periodic contraction, during the process of cell adhesion. The experimental technique used to obtain the data was traction force microscopy (TFM), this technique consists in tracking the traction produced by a cell adhered in a elastic surface of polyacrylamide gel (rigidity 9.6kPa). The possible application of this study is to differentiate phenotypes or disease states based on mechanical experimental data. The traction field is calculated based on the motion of fluorescent particles in a elastic surface of polyacrylamide gel. Mapping the cell (neonatal cardiomyocytes) adhesion area and calculating the traction field. There is a specific distribution, typically characterized by a contraction signal region and a random signal, corresponding to the focal adhesions of the cell. In order to perform periodic signal strength field analysis, we used the wavelet transform for the possibility of obtaining frequency and time domain signals at various scales. The results show that the frequency in the regions of contraction remains stable over time, having a high correlation between them.